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| Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C.. |
| Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also... |
| Tipo: Text |
Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf |
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| Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika. |
| Microplastics (<5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges... |
| Tipo: Text |
Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf |
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| Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick. |
| This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf |
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| Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre. |
| Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative... |
| Tipo: Text |
Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf |
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